Data Science Fellow — Organoid Biochemistry

The posting, as Axle wrote it

(ID: 2026-3888)

Axle is a bioscience and information technology company that offers advancements in
translational research, biomedical informatics, and data
science applications to research centers and healthcare
organizations nationally and abroad. With experts in
biomedical science, software engineering, and program
management, we focus on developing and applying research
tools and techniques to empower decision-making and
accelerate research discoveries. We work with some of the
top research organizations and facilities in the country
including multiple institutes at the National Institutes of
Health (NIH).

Benefits We Offer

• 100% Medical, Dental & Vision Coverage for Employees
• Paid Time Off and Paid Holidays
• 401K match up to 5%
• Educational Benefits for Career Growth
• Employee Referral Bonus

Flexible Spending Accounts

• Healthcare (FSA)
• Parking Reimbursement Account (PRK)
• Dependent Care Assistant Program (DCAP)
• Transportation Reimbursement Account (TRN)

Position Overview

The Postdoctoral Fellow will help establish the laboratory’s core capacity for protein-focused biological support, connecting organoid phenotype and optical measurements to molecular mechanisms. The Fellow will implement two complementary platforms: Amplicon/Protein Bead Display (APB-Display) for rapid production, screening, and quantitative characterization of protein or nanobody libraries; and SMA/SMALP or related membrane-active-polymer workflows for detergent-free extraction of membrane proteins and associated lipids into native nanodiscs. The position serves as the principal biochemical resource for the two existing optical-methods postdocs and works closely with the chemical-spectroscopy postdoc on toxicant-induced changes in proteins, membranes, and lipid environments. The appointment is intended to prepare the Fellow for an independent research career in academia, government, or industry.

Key Responsibilities

• Biochemical support. Establish routine molecular-biology, protein-expression, purification, conjugation, and biochemical-assay support for organoid projects, including recombinant protein and nanobody production in bacterial, yeast, cell-free, or mammalian systems as appropriate.
• APB-Display implementation. Implement APB-Display using dual-functional hydrogel beads, pooled DNA libraries, particle-templated emulsification, emulsion PCR, emulsion in-vitro transcription/translation, SNAP-mediated protein capture, fluorescence-based expression and binding measurements, FACS sorting, and sequencing-based readout.
• Library design. Develop focused nanobody and affinity-reagent libraries informed by in-silico design, biological targets, chemical-toxicity hypotheses, and needs identified by the imaging and spectroscopy researchers.
• Quantitative screening. Implement APB-SortSeq for rapid single-concentration screening and APB-TiteSeq for quantitative concentration-series measurements of expression and binding affinity; establ

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Preferred Qualifications

• Experience with nanobodies, antibody engineering, display technologies, pooled libraries, FACS, next-generation sequencing, cell-free protein synthesis, microfluidics, or hydrogel beads.
• Experience with styrene-maleic-acid polymers, SMALPs, native nanodiscs, membrane-protein purification, lipidomics, quantitative proteomics, mass spectrometry, or electron microscopy sample preparation.
• Experience with organoids, stem-cell-derived models, toxicology, chemical exposures, fluorescence imaging, or multi-omics integration.
• A strong record of method development, troubleshooting, collaborative publications, and independent experimental execution.

Disclaimer: The above description is meant to illustrate the general nature of work and
level of effort being performed by individuals assigned to this position or job description. This is not
restricted as a complete list of all skills, responsibilities, duties, and/or assignments required. Individuals
may be required to perform duties outside of their position, job description or responsibilities as needed.

The diversity of Axle’s employees is a tremendous asset. We are firmly committed to providing equal opportunity
in all aspects of employment and will not tolerate any illegal discrimination or harassment based on age, race,
gender, religion, national origin, disability, marital status, covered veteran status, sexual orientation,
status with respect to public assistance, and other characteristics protected under state, federal, or local law
and to deter those who aid, abet, or induce discrimination or coerce others to discriminate.

Accessibility: If you need an accommodation as part of the employment process please contact: careers@axleinfo.com

This role has a market-competitive salary with an anticipated base compensation range listed below. Actual salaries will vary depending on a candidate’s experience, qualifications, skills, and location.

Salary Range
$90,000 — $100,000 USD